. 1998 Apr;180(8):2057–2062. doi: 10.1128/jb.180.8.2057-2062.1998

TABLE 1.

B. subtilis strains and plasmids used

Strain or plasmid	Genotype and phenotype	Source or reference
B. subtilis strains
PS832^a	Trp⁺ revertant of strain 168	Laboratory stock
PS2488^a	katA::cat Cm^r	4
PS2558^a	katX::cat Cm^r	4
PS2573^a^,^c	katA::katA-lacZ Cm^r	YB2003→PS832^d
PS2663^a	katX::erm Em^r	pCM::Erm→PS2558
PS2664^a	katA::cat katX::erm Cm^r Em^r	PS2663→PS2488
PY79^b	Wild type	43
RL831^b	spoIIIG::kan Km^re	R. Losick
SC137^b	spoIIGB::erm Em^r	S. Cutting
SC500^b	spoIIIGΔ1	S. Cutting
SC829^b	spoIVCB23	S. Cutting
IB427^a	katX::katX-lacZ Cm^r	pIB426→PS832
IB429^b	katX::katX-lacZ spoIIAC Cm^r	IB427→SC1159
IB430^b	katX::katX-lacZ spoIIGB Cm^r Em^r	IB427→SC137
IB431^b	katX::katX-lacZ spoIIIG Cm^r	IB427→SC500
IB432^b	katX::katX-lacZ spoIVCB Cm^r	IB427→SC829
IB434^b	katX::katX-lacZ Cm^r	IB427→PY79
IB435^b	katX::katX-lacZ spoIIIG [pSDA4] Cm^r Km^r	pSDA4→IB431
IB436^b	katX::katX-lacZ spoIIIG [pDG298] Cm^r Km^r	pDG298→IB431
SC1159^b	spoIIAC1	S. Cutting
IB439^a	amyE::katX-lacZ Cm^r	pIB437→PS832
IB441^a	amyE::katX-lacZ spoIIIG::kan Cm^r Km^r	RL831→IB439
IB446^a	katA::erm Em^r	pCm::Erm→PS2488
IB447^a	katA::erm katX::katX-lacZ Cm^r Em^r	IB427→IB446
YB2003^c	katA::katA-lacZ Cm^r	2
Plasmids
pCm::Erm	Amp^rf Em^r	35
pCR2.1	Amp^r Km^r	Invitrogen
pDG268	Amp^r Cm^r	37
pDG298	Km^r, carries spoIIIG (ς^G) under P_spac control	40
pIB426	Amp^r Cm^r, translational katX-lacZ fusion in pJF751	This work
pIB437	Amp^r Cm^r, translational katX-lacZ fusion in pDG268	This work
pIB449	1,181 bp of katX coding and upstream sequence in pCR2.1	This work
pJF751	Amp^r Cm^r	10
pSDA4	Amp^r Km^r, carries spoIIAC (ς^F) under P_spac control	32

Genetic background is PS832.

Genetic background is PY79.

katA mutant.

The source of the donor DNA in transformation is to the left of the arrow, and the recipient is to the right.

Km^r, kanamycin resistance.

Amp^r, ampicillin resistance.