FIG. 5.
PCR analysis of the ΔrecBCD::Plac-red allele. Lanes: 1, 1-kb DNA ladder standards (Gibco-BRL); 2 to 4, PCR products generated with primers 7, 8, and 9 (Fig. 4) from JC9387 (recBC sbcBC), KM20 (ΔrecBCD::Plac-red sbcBC), and the control reaction (no cells), respectively; 5 to 7, PCR products generated with primers 10, 11, and 12 (Fig. 4) from JC9387 (recBC sbcBC), KM20 (ΔrecBCD::Plac-red sbcBC), and the control reaction (no cells), respectively. The positions of 1- and 2-kb DNA standards are shown by arrows.