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. 2023 Apr 3;41(12):1787–1800. doi: 10.1038/s41587-023-01718-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (a) Violin plots of the expression levels of markers of cycling cells (TOP2A, PLK1) in the cardiomyocyte (CM) clusters obtained from the scRNA-seq analysis of epicardioids at days 15 and 30. (b) Average expression ratio of the adult to fetal cardiac troponin I isoforms (TNNI3/TNNI1) in ventricular CM (vCM) clusters obtained from scRNA-seq analysis of epicardioids at days 15 and 30. (c) Violin plots of the expression levels of genes related to CM Ca²⁺ handling (ATP2A2, PLN, SLC8A1) in vCM clusters; cells from epicardioids at day 15 and 30 are shown in red and blue, respectively. (d) Violin plots of the expression levels of markers of pacemaker CMs (SHOX2, HCN4) in the CM clusters obtained from the scRNA-seq analysis of epicardioids at days 15 and 30.