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. 2023 Dec 11;14(12):815. doi: 10.1038/s41419-023-06352-4

Fig. 4. Mint3 promotes HSP70 expression in TNBC in an in vivo-specific manner.

Fig. 4

A–D mRNA levels of APBA3 (encoding Mint3) (A, C) and HSPA1A (encoding HSP70) (B, D) in MDA-MB-231 (A, B) and MDA-MB-468 (C, D) ishMint3 cells and tumors treated with or without doxycycline (DOX). Data are presented as mean ± SEM and were analyzed using the Mann–Whitney U-test for MDA-MB-231 cells and MDA-MB-231 and MDA-MB-468 tumors, or the Welch’s t-test for MDA-MB-468 cells. **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. E, F Protein levels of Mint3 and HSP70 in MDA-MB-231 ishMint3 cells (E) and tumors of these cells (n = 5 per group) (F) treated with or without doxycycline (DOX). G, H Protein levels of Mint3 and HSP70 in MDA-MB-468 ishMint3 cells (G) and tumors of these cells (n = 5 per group) (H) treated with or without doxycycline (DOX). I, J Immunostaining for cleaved caspase-3 in tumors from MDA-MB-231 cells 24 h after one-shot chemotherapy with the vehicle, DXR, or PTX in combination with the HSP70 inhibitor VER 155008 (25 mg/kg bw). I Representative images. J Cleaved caspase-3-positive areas were counted in the tumor sections. n = 18 from six tumors per group. The data are presented as the mean ± SEM and were analyzed using the Mann–Whitney U-test. ****p < 0.0001. NS not significant.