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. 2023 Dec 11;14(12):815. doi: 10.1038/s41419-023-06352-4

Fig. 5. HIF-1 promotes HSP70 expression in TNBC in an in vivo-specific manner.

Fig. 5

A Protein level of HIF-1α, Mint3, and HSP70 in MDA-MB-231-expressing doxycycline-inducible shRNAs against HIF-1α (ishHIF-1α) cells treated with or without doxycycline (DOX; 1 μg/ml) for three days. B Protein levels of HIF-1α, Mint3, and HSP70 in MDA-MB-231 ishHIF-1α tumors administered with or without DOX (2 mg/mL in tap water) for 3 days. n = 5 per group. C, D mRNA levels of HSPA1A (C) and HIF1A (D) in MDA-MB-231 ishHIF-1α tumors administered with or without doxycycline for 3 days. n = 8 per group. Data represent mean ± SEM and were analyzed using the Mann–Whitney U-test. **p < 0.01, ***p < 0.001. E–G Immunostaining for Mint3, HIF-1α, and HSP70 in TNBC specimens (n = 126). E Representative immunostaining images. Bar = 50 µm. F The correlation between Mint3 and HSP70 protein expression was statistically analyzed using the chi-square test. G The correlation between HIF-1α and HSP70 protein expression was statistically analyzed using Fisher’s exact test.