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. 2023 Dec 11;14(12):815. doi: 10.1038/s41419-023-06352-4

Fig. 6. Mint3 activates HSF-1 via the mTOR signaling pathway in TNBC tumors.

Fig. 6

A–F mRNA levels of HSF1 target genes (HSPA1B, HSPA6, and DNAJB1) in MDA-MB-231 (A–C) and MDA-MB-468 (D–F) ishMint3 cells and tumors treated with or without doxycycline (DOX). Data are presented as mean ± SEM and were analyzed using the Mann–Whitney U-test for MDA-MB-231 cells and MDA-MB-231 and MDA-MB-468 tumors, or the Welch’s t-test for MDA-MB-468 cells. *p < 0.05, ***p < 0.001, ****p < 0.0001. NS: not significant. G Immunoblotting of HSF-1 and its upstream signaling-related proteins in MDA-MB-231 ishMint3 tumors treated with or without DOX for three days. n = 3 per group. H Immunoblotting of HSF-1 and mTOR signaling-related proteins in MDA-MB-468 ishMint3 tumors treated with or without DOX for 3 days. n = 3 per group. I Immunoblotting of Mint3, HSF-1, and mTOR signaling-related proteins in MDA-MB-231 tumors treated with or without the mTOR inhibitor AZD8055 (10 mg/kg) for 3 days. n = 3 per group.