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. 2023 Dec 11;14:8038. doi: 10.1038/s41467-023-43892-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The bending modulus was analyzed for DPPC (a) and DOPC bilayers (b) based on unperturbed coarse-grained MD simulations in the absence of cholesterol (gray bars), in presence of 40% cholesterol, with blocked cholesterol flipping between the layers (noflip), and for simulations with blocked cholesterol flipping and restrained lateral cholesterol diffusion (restr.). Displayed are mean values and errors as 95% confidence intervals employing parametric bootstrapping (N = 50,000 statistically independent samples) assuming Gaussian distributions of the mode-dependent amplitudes⁷⁸.