Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 12;13:22000. doi: 10.1038/s41598-023-49199-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SIRT6 prevents tumor cells from Delta16HER2-induced G2/M arrest and senescence. (A) Ex vivo cell cycle analysis of cell suspensions derived from Delta16HER2 and Delta16HER2/SIRT6-OE tumors at 20 weeks of age (top panels) and at 30 weeks of age (bottom panels). DNA content was evaluated by flow cytometry using propidium iodide (PI) staining (n = 3) and then analyzed using Dean-Jett-Fox algorithm in FlowJo software. Percentages of cells in each cell cycle phase are summarized in each panel. (B) Immunoblot image and quantification of Cyclin D1 and Cyclin E levels normalized to β-Actin in tumors of either 20- (top) or 30-week-old (bottom) Delta16HER2 and Delta16HER2/SIRT6-OE mice (n = 4). (C) mRNA expression of Trp53, Cdkn2a and Cdkn1a genes normalized to β-Actin mRNA level in tumors of 30-week-old Delta16HER2 controls and Delta16HER2/SIRT6-OE mice (n = 4/group). (D) Detection of senescence-associated β-galactosidase (SA-β-Gal) activity in frozen tumors of 30-week-old Delta16HER2 and Delta16HER2/SIRT6-OE (n = 3). Quantification is expressed as % of SA-β-Gal positive area (blue) with respect to the total section area. Scale bar, 25 mm. In (B–D) ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed unpaired t test). Error bars represent SD. See also Fig. S2.