Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 11;14:8212. doi: 10.1038/s41467-023-43633-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A Upstream (left) and downstream (right) nucleotide preference in ADAR-specific editing. Editing levels correspond to adenosines along both double-stranded constructs, but As near the loop were excluded. Data is visualized using box-and-whisker plots, where the central line represents the median, the box edges depict the interquartile range, and the whiskers show 1.5 times the interquartile range. B Constructs characterized by a systematic C, A or G base opposite to A along the stem. Line charts show the effect of the different mismatches on editing. The Δ editing level on the y-axis represents the change in the editing level of an adenosine, normalized to the double-stranded construct. Fitted curves depict the LOESS fit of Δ editing with a span of 0.07. C Subset of G-mismatching bases that neighbor the edited sites. Left—Graphical scheme. Right—On the heatmap, the x-axis shows the distance of the disruptions to the A site while the y-axis represents the base to which a G is opposite. D Effect of 3-nt mismatch running through the stem on adenosine within the GA sequence context in ADAR2-expressing cells. Mismatches differentially located in each construct get centered at 0 on the x-axis. The Δ editing on the y-axis represents the change of the editing of an adenosine, normalized to the perfect double-stranded construct. The box plot depicts the distribution of Δ editing levels per distance. Data is shown as Fig. 5A. E Scheme of arRNAs targeting endogenous SMAD4 transcript. (1) The ‘Empty Vector’ as negative control. (2) ‘Perfect ds’ construct is a 151-bp oligo complementary to the transcript. (3) ‘3 bp mismatch’ construct consists of an arRNA as in (2) but containing a 3-nucleotide mismatch opposite to the target A site. (4-5) ‘G-G and G-A mismatch’ constructs consist of an arRNA as in (2) but including a G-G and G-A mismatches one nucleotide upstream from the A site. F Quantification showing the editing levels on the adenosine of the SMAD4 in ADAR2-expressing cells, presented as mean ± s.e.m. (n = 3 independent experiments) and evaluated with a two-tailed t-test.