Fig. 3. Loss of IL-31RA has no effect on Th2 responses and goblet cell hyperplasia during house dust mite (HDM)-induced allergic asthma.

a Quantification of Th2 cytokine transcripts including IL-4, IL5, and IL-13 in the whole lung tissue of wild-type and IL-31RA^-/- mice treated with saline (n = 6) or HDM (n = 6) using RT-PCR. Data are shown as mean $\pm$SEM. One-way ANOVA was used. b Quantification of chemokines and inflammatory cytokines including CCL11, CCL24, and IL-10 in the whole lungs of wild-type and IL-31RA^-/- mice treated with saline (n = 6) or HDM (n = 6) using RT-PCR. Data are shown as mean $\pm$SEM. One-way ANOVA was used. c Quantification of Th2 response-associated gene transcripts including CHI3L3, ARG1, and FIZZ1 in the total lungs of wild-type and IL-31RA^–/– mice treated with saline (n = 6) or HDM (n = 6) using RT-PCR. Data are shown as mean $\pm$SEM. One-way ANOVA was used. d Quantification of GOB5 and MUC5AC gene transcripts in the whole lungs of wild-type and IL-31RA^–/– mice treated with saline (n = 6) or HDM (n = 6) using RT-PCR. Data are shown as mean $\pm$SEM. One-way ANOVA was used. e Concentration of cytokines IL-4, IL-13, IL-5, IL-6, IFNγ, TNF-α and IL-10 in the BAL fluids of wild-type and IL-31RA^–/– mice treated with saline (n = 6) or HDM (n = 6) using the cytometric bead assay LEGENDplex. Data are shown as mean $\pm$SEM. One-way ANOVA was used. At least two independent experiments produced similar results. Source data are provided as a Source Data file.