Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 11;6:1253. doi: 10.1038/s42003-023-05546-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, The HAB tag on TRβ includes an HA epitope and site (target lysine underlined) for biotinylation by BirA ligase (expressed by a Rosa26^BirA allele). b TRβ-HAB transcripts in pituitary RNA detected by PCR. c Expression of tagged receptors in pituitary sections detected by immunostaining for TRβ2. Thrb-KO, TRβ-deficient control. DAPI, nuclear stain shows tissue background. d Immunofluorescence analysis of anterior pituitary sections for tagged (HAB;BirA) and non-tagged receptors (control, BirA) showing nuclear signal detected with TRβ2 immunostaining. Arrowheads, representative nuclei. Alpha-tubulin cytoskeletal stain indicates cell outlines. e, f Normal thyroid gland morphology and anterior pituitary dimensions in HAB;BirA mice. Thyroid areas were measured on plastic sections. Groups, 4–6 adult males; mean ± SD (P > 0.2, one-way ANOVA). Anterior pituitary dimensions were measured for half-lobe areas in cryosections. Groups 4–6 males; mean ± SD (P > 0.16, one-way ANOVA). A.U., arbitrary units. g Serum total T4, T3 and TSH in adult males; mean ± SD. Groups, 8–12; No significant differences were detected between genotypes (P > 0.1, one-way ANOVA). h Pituitary gene RNA expression analysis by qPCR. Groups, 5–6 adult males; mean ± SD. No significant differences between genotypes (P > 0.1, one-way ANOVA).