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. 2023 Dec 11;14:8103. doi: 10.1038/s41467-023-43765-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Experimental setup of the screening assay. Briefly, neutrophils from human blood were isolated and plated on a 384-well plate, treated with one of the 1000 compounds from a library of known bioactives and stimulated with fMLP. Neutrophil motility was then recorded simultaneously in 64 wells of a 384-well plate for one hour (8 s between frames) using ComplexEye. Afterwards the motility was analyzed via single cell tracking. b Data represent 1000 movies, ~800 tracks/movie and show the impact of 1000 compounds screened in 17 rounds, each round with three controls (PBS, DMSO and fMLP or fMLP/DMSO). The heatmap shows each round with 64-wells with the relative speed of imaged neutrophils indicated as color code compared to the fMLP-control in that run (artificially set to 1.0). Compounds that reduced the speed are shown in green-blue (low speed). Indicated gray wells were non-evaluable due to production residues of the 384-well plates inhibiting clear sight of the cells. Source data are provided as a Source Data file.