Fig. 6.

Immunohistochemical detection of the distribution of YAP and GLI2α in TAA-induced liver fibrosis. A Representative fluorescence microscopy of the distributions of YAP and GLI2α in liver sections using the indicated antibodies: anti-YAP (red), anti-GLI2α (green) and nuclei were stained with 4′, 6-diamidino-2-phenylindole DAPI (blue). The corresponding overlay of YAP and GLI2α is shown in panel (YAP + GLI2α) and the corresponding overlays of the three signals is shown in panel (merge). Scale bar = 50 µm. B Quantification of relative fluorescence intensity of YAP and GLI2α in control and TAA-induced liver fibrosis. C Percent of YAP/GLI2α nuclear colocalization in control and TAA-induced liver fibrosis. The fluorescence intensity of each section was measured by ImageJ. *p < 0.05, n = 5, means ± SD