Table 2.

The observed and predicted responses of the Box Behnken design

Run order	pH	Inoculum size (% v/v)	Incubation period (days)	Observed response	Predicted response
1	6.5	1	1	0	2.50
2	9	2.5	1	16	15.63
3	6.5	2.5	3.5	18	19.44
4	6.5	2.5	3.5	19	19.44
5	9	2.5	6	0	0.88
6	6.5	2.5	3.5	20	19.44
7	4	2.5	6	20	19.13
8	4	2.5	1	16	13.87
9	6.5	4	1	0	0
10	6.5	1	6	20	21.25
11	9	1	3.5	17	14.82
12	6.5	2.5	3.5	20	19.44
13	6.5	2.5	3.5	19	19.44
14	4	1	3.5	24	23.07
15	9	4	3.5	16	15.82
16	6.5	4	6	0	1.25
17	4	4	3.5	22	24.07
$\begin{array}{cc}\hfill \text{CPC activity}=& -26.81167+1.15000\ast \text{A}+11.30000\ast \text{B}+20.00111\ast \text{C}\hfill \\ \hfill -& 0.80000\ast \text{A}\ast \text{C}-3.13333\ast \text{B}\ast \text{C}-1.13111\ast \text{C2}\hfill \\ \hfill \end{array}$ (2)