Fig. 2.

Graphical illustration of the in vivo experiment. (A) Schematic schedule, (B) operation procedure, (C) a representative Doppler flowmetry during the operation, and (D) region of interest for histologic studies are presented. In (B), circled “1”, “2”, and “3” represent the steps of right common carotid artery ligature, left common carotid artery ligature, and blood withdrawal via a femoral artery catheter, respectively, during BCCAO/H operation. In (D), the red box (300 µm in width) in hippocampal cornu ammonis 1 point out a region of interest for histologic analyses. POD, postoperative day; BCCAO/H, bilateral common carotid artery occlusion and hypotension; NORT, novel object recognition test; BMT, Barnes maze test; Y-MT, Y-maze test; PAT, passive avoidance test; VO, vessel occlusion.