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SDS-polyacrylamide gel electropherogram of the fractions of the following purification steps of PPi-PFK: molecular size standard (lane 1), crude extract (40 μg of protein; lane 2), heat-treated extract (40 μg of protein; lane 3), anion-exchange chromatography product (20 μg of protein; lane 4), hydrophobic interaction chromatography product (20 μg of protein; lane 5), and gel filtration product (3 μg of protein; lane 6).
