Figure 9.

Protective Effects of NMT2 in the Heart Are Mediated by CaMKII-Related Histone H3 Acetylation Signaling

Immunoblot analysis for CaMKII phosphorylation, (A) HDAC4 phosphorylation, and (B) histone H3 acetylation in cardiac NMT2-overexpressing mice after TAC (n = 5-8). (C) KEGG analyses of RNA sequencing from the left ventricle. Log q-values, rich ratio, and top 10 KEGG enrichment pathways of TAC-operated mice receiving AAV9-NMT2 in comparison to TAC-operated AAV9-CTRL–injected mice are shown in a bubble plot. The size of the circle depicts the number of genes annotated to each KEGG pathway. (D) The proposed model of a cardioprotective role of N-myristoylation through NMT2 in cardiac myocytes. All data are presented as mean ± SEM. ∗P < 0.05 and ∗∗∗P < 0.001 by 1-way analysis of variance with Tukey’s post hoc analysis. Abbreviations as in Figures 1, 5, and 8.