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. 2023 Dec 11;29(12):583–595. doi: 10.1089/ten.tec.2023.0122

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© Ana Luísa Cartaxo et al. 2023; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — Workflow of the proposed two-step production of a combined product consisting of expanded SkMDCs attached to TIPS PLGA microcarriers. First, SkMDCs are expanded in a planar system using FBS-based culture medium. These cells are then combined with biocompatible and biodegradable TIPS PLGA microcarriers in a VWBR. The combined xeno-free product is obtained by culture of VWBR in an intermittent agitation scheme, using hPL-based culture medium for 48 h. FBS, fetal bovine serum; hPL, human platelet lysate; PLGA, poly(lactic-co-glycolic acid); SkMDC, skeletal-derived muscle cells; TIPS, thermally induced phase separation; VWBR, Vertical-Wheel^® bioreactor. Color images are available online.