FIG. 2.

Effect of upstream sequences on pilEp₁ transcription in N. gonorrhoeae. CAT levels were measured in cell extracts prepared as described in Materials and Methods from gonococcal reporter strains containing pilEp₁::cat cassettes with wild-type (WT) or substituted upstream sequences (explained in the legends to Fig. 1 and 4). The results shown are averages of at least four separate experiments, and error bars represent 1 standard deviation.