FIG. 4.

Comparison of the sequence upstream of pilEp₁ with the sequence upstream of the λ P_L1 promoter (14). The −35 regions of pilEp₁ and λ P_L1 are contained within the open boxes. The −10 and −35 sequences associated with λ P_L2 are in italics and underlined, with the TSP denoted by the bent arrow. The nucleotides contained within the hatched box have been shown to function as an UP element through the binding of the α subunit of RNAP. Asterisks denote identical nucleotides shared between the pilEp₁ and λ P_L sequences within this region. IHF-binding core consensus sequences are underlined in boldface. Nucleotides below the double-headed arrow were deleted in the construction of the Δ −38−77 and P_L-pilEp₁ reporter derivatives, resulting in the relocation of either the gonococcal putative UP element or the λ P_L UP element directly upstream of nucleotide −37.