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. 2023 Dec 12;42:336. doi: 10.1186/s13046-023-02902-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Contact with PC cells reshapes the transcriptional profile of endothelial cells. A Fold differences of mRNAs of angiogenesis-related genes, between HUVEC (yellow bars) or HAEC (red bars) cocultured with wild type DU145 versus HUVEC or HAEC, respectively. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. B Fold differences of mRNAs of angiogenesis-related genes between HUVEC (yellow bars) or HAEC (red bars) cocultured with wild type PC3 versus HUVEC or HAEC, respectively. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. C Fold differences of mRNAs of angiogenesis-related genes between HUVEC cocultured with wild type DU145 (yellow bars), or PC3 (green bars), versus HUVEC alone, and between HAEC cocultured with wild type DU145 (red bars), or PC3 (blue bars) versus HAEC alone. Genes whose regulation was shared by both endothelial cell types are represented. A significant threshold of a twofold change in gene expression corresponded to p < 0.001. Experiments were performed in duplicate. D Western blot analysis of ANG, CXCL9, EDN1 and TGFB2 protein expression in HUVEC cocultured with DU145 (left side of the panel) or PC3 cells (right side of the panel). Representative images of three experiments