FIG. 3.

PolyP accumulation depends on amino acid (aa) concentration in the growth medium. E. coli MG1655 was grown in minimal medium containing 0.1 mM P_i (20 μCi of ³²P_i per ml) and the following concentrations of each of the 20 amino acids: 2 (A), 20 (B), or 0 (C) μg/ml. (D) Cells grown in minimal medium with excess P_i (2 mM). Growth and polyP content of the cells were measured as described in the legend to Fig. 1.