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Effect of amino acid supplementation on AP induction. E. coli MG1655 was grown in a Pi-limited (0.1 mM) minimal medium without (A and B) or with (C and D) the addition of 2 μg of each of the 20 amino acids per ml. Panels A and C highlight the initial (up to 4-h) growth period and corresponding AP activity of cells represented in panels B and D, respectively. Note the scales on the y-axes.
