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Effects of ΔrelA on growth, polyP accumulation, and (p)ppGpp levels. The wild type and a corresponding relA mutant (MG1655 ΔrelA251) were grown in Pi-limited minimal medium containing 2 μg of each of the 20 amino acids per ml and analyzed for polyP accumulation (A and B), and ppGpp and pppGpp levels (C and D).
