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Effects of phoB on polyP accumulation and (p)ppGpp levels. MG1655 wild-type and MG1655 phoB::Tn5 mutant strains were grown in minimal medium containing 0.4 mM Pi and 20 μg of amino acids per ml. At an OD540 near 0.2, SHX was added to induce amino acid starvation. At the times indicated after the addition of SHX, samples were collected and analyzed for polyP (A and C) and (p)ppGpp (B and D).
