TABLE 2.
Strain | Relevant genotype | PolyP (nmol/mg of protein) |
---|---|---|
AT833 | K10 wild type | 10.8 |
AT13 | K10 phoB63 | 1.8 |
AT854 | K10 ΔphoU | 7.7 |
AT857 | K10 phoU35 | 15 |
AT280 | K10 phoR68 | 16.9 |
AT776 | K10 phoR69 | 17.6 |
AT884 | GP3 phoB::Tn5 | 1.8 |
AT25 | LEP1 phoB23 | 1.8 |
AT30 | G206 phoB62 | 1.9 |
NR21 | W3110 wild type | 115 |
AT401 | W3110 phoR creC | 0.1 |
CF1648 | MG1655 wild type | 48 |
NR2 | MG1655 phoB::Tn5 | 0.3 |
MOPS-buffered minimal medium, with 0.1 mM Pi and 2 μg of each amino acid per ml, was used. The OD540 values at the time of sampling were near 0.3.