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The anti-mycobacterial activity of LysB was determined by plate lysis assay on MB 7H11 medium plate in the (A) presence or (B) absence of Tween-80. Anti-mycobacterial activity is indicated by the formation of a lytic zone. In the presence of Tween-80, the clear spot zone was observed at 3 µg/mL. In the absence of Tween-80, the concentration up to which a clear spot zone was observed increased and was visible at 6 µg/mL.
