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. 2023 Oct 9;11(6):e01035-23. doi: 10.1128/spectrum.01035-23

TABLE 1.

Identifying LPMO-related proteins encoded in the P. putredinis NO1 genome a

Coding region GenBank accession Protein ID Identified by searching approach InterPro annotation
Sequence Domain Structure
FUN_000653-T1 CAI7987917.1 PutMoA AA16 LPMO
FUN_000713-T1 CAI7987978.1 PutMoB Rho factor associated
FUN_002573-T1 CAI7991617.1 PutMoC
FUN_002890-T1 CAI7992277.1 PutMoD AA16 LPMO
FUN_002962-T1 CAI7992399.1 PutMoE
FUN_003190-T1 CAI7992922.1 PutMoF Ferritin-like
FUN_003535-T1 CAI7993628.1 PutMoG AA13 LPMO
FUN_003783-T1 CAI7994168.1 PutMoH
FUN_006366-T1 CAI7999797.1 PutMoI AA9 LPMO
FUN_006413-T1 CAI7999893.1 PutMoJ AA9 LPMO
FUN_006553-T1 CAI8000144.1 PutMoK
FUN_007242-T1 CAI8001774.1 PutMoL AA9 LPMO
FUN_007666-T1 CAI8002525.1 PutMoM AA9 LPMO
FUN_008106-T1 CAI8003467.1 PutMoN AA9 LPMO
FUN_009239-T1 CAI7992001.1 PutMoO
FUN_010012-T1 CAI8003342.1 PutMoP
a

Coding regions of proteins related to LPMOs identified through genome searching approaches with the sequence of an A. niger AA9 LPMO (E-value cutoff = 1 × 10−5), the Pfam AA9 HMM (significance threshold = 0.01), and the structure of the A. niger AA9 LPMO (lowest percentage match = 50%) and which were not annotated as AA9 CAZymes by dbCAN. InterPro annotations were retrieved where possible