TABLE 2.
Identifying laccase-related proteins encoded in the P. putredinis NO1 genome a
| Coding region | GenBank accession | Protein ID | Identified by searching approach | InterPro annotation | ||
|---|---|---|---|---|---|---|
| Sequence | Domain | Structure | ||||
| FUN_000263-T1 | CAI7987524.1 | PutLacA | ✔ | – | ||
| FUN_000580-T1 | CAI7987844.1 | PutLacB | ✔ | Phospodiesterase | ||
| FUN_000646-T1 | CAI7987911.1 | PutLacC | ✔ | – | ||
| FUN_000759-T1 | CAI7988026.1 | PutLacD | ✔ | ✔ | Multi-copper oxidase | |
| FUN_000832-T1 | CAI7988099.1 | PutLacE | ✔ | – | ||
| FUN_001183-T1 | CAI7988671.1 | PutLacF | ✔ | – | ||
| FUN_001583-T1 | CAI7989479.1 | PutLacG | ✔ | Salt tolerance regulator | ||
| FUN_002249-T1 | CAI7990863.1 | PutLacH | ✔ | ✔ | ✔ | AA1 multi-copper oxidase |
| FUN_002874-T1 | CAI7992258.1 | PutLacI | ✔ | – | ||
| FUN_003732-T1 | CAI7994085.1 | PutLacJ | ✔ | – | ||
| FUN_003828-T1 | CAI7994234.1 | PutLacK | ✔ | – | ||
| FUN_004259-T1 | CAI7995254.1 | PutLacL | ✔ | Nucleoside hydrolase | ||
| FUN_004616-T1 | CAI7995870.1 | PutLacM | ✔ | – | ||
| FUN_004739-T1 | CAI7996089.1 | PutLacN | ✔ | Fumarylacetoacetate hydrolase family | ||
| FUN_005132-T1 | CAI7997298.1 | PutLacO | ✔ | ✔ | AA1 multi-copper oxidase | |
| FUN_005520-T1 | CAI7998008.1 | PutLacP | ✔ | Diacylglycerol acyltransferase | ||
| FUN_006244-T1 | CAI7999594.1 | PutLacQ | ✔ | – | ||
| FUN_006620-T1 | CAI8000270.1 | PutLacR | ✔ | Fumarylacetoacetate hydrolase family | ||
| FUN_006720-T1 | CAI8000684.1 | PutLacS | ✔ | Glycosyltransferase 90 | ||
| FUN_007228-T1 | CAI8001746.1 | PutLacT | ✔ | – | ||
| FUN_007508-T1 | CAI8002246.1 | PutLaU | ✔ | Pex2 | ||
| FUN_008329-T1 | CAI8004041.1 | PutLacV | ✔ | ATPase-related | ||
| FUN_009491-T1 | CAI7995256.1 | PutLacW | ✔ | Helicase | ||
a
Coding regions of proteins related to laccases identified through genome searching approaches with the sequence of an A. niger AA1 laccase (E-value cutoff = 1 × 10−5), the bespoke laccase and multicopper oxidase HMM constructed from sequences from the laccase engineering database (significance threshold = 0.01), and the structure of the A. niger AA1 laccase (lowest percentage match = 30%) and which were not annotated as AA1 CAZymes by dbCAN. InterPro annotations were retrieved where possible