TABLE 3.
Growth parameters of phage-resistant mutants a
| Strain | Mutated gene | Plasmid | OD600 b | g b |
|---|---|---|---|---|
| PAO1 | – | pGM931 | 1.5 ± 0.0 | 81.5 ± 2.3 |
| PAER5b | wapH | pGM931 | 1.0 ± 0.1 | 107.8 ± 4.0 |
| pGM-wapH | 1.5 ± 0.0 | 79.1 ± 1.9 | ||
| PAER6b | galU | pGM931 | 1.1 ± 0.0 | 110.1 ± 6.9 |
| pGM-galU | 1.4 ± 0.0 | 75.5 ± 1.4 | ||
| PADR6 | wzy | pGM931 | 1.4 ± 0.0 | 78.9 ± 2.0 |
| pGM-wzy | 1.4 ± 0.0 | 75.5 ± 1.8 | ||
| PAER10b | algC | pGM931 | 1.3 ± 0.0 | 80.9 ± 2.2 |
| pGM-algC | 1.4 ± 0.0 | 76.8 ± 1.4 |
a
Growth in LB with 300 µg/mL carbenicillin and 0.2% arabinose in 96-well plates as determined by measuring the OD600 at 30-min intervals.
b
Average (N = 6) and standard deviation of the optical density (OD600) reached after 24 h at 37°C and of the generation time (g; in min) in exponential phase. The difference in both parameters evaluated with ANOVA test is significant (P = 1.1 × 10−25 and 2.6 × 10−27 for OD600 and g, respectively).