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. 1998 Apr;180(8):2212–2219. doi: 10.1128/jb.180.8.2212-2219.1998

TABLE 1.

Specific activities of FGD expressed from recombinant plasmids in E. coli BL21(DE3)

E. coli BL21(DE3) carrying: Mean FGD sp act (μmol of F420 reduced/min/mg of protein) ± SD
2 ha
15 hb
No IPTG With IPTG No IPTG With IPTG
pEP7000 0.014 ± 0.002 0.018 ± 0.002 0.030 ± 0.003 0.011 ± 0.001
pEP5000 0.011 ± 0.002 0.010 ± 0.001 0.017 ± 0.002 0.005 ± 0.001
pEP3000 0.173 ± 0.018 0.254 ± 0.012 0.410 ± 0.014 0.187 ± 0.015
pEP1300 0.238 ± 0.014 0.277 ± 0.024 0.523 ± 0.033 0.224 ± 0.022
pEP1088 0 0 0.015 ± 0.001 0
pEP1300::Kanr 0 0 0 0
a

Cells were harvested 2 h after addition of IPTG. 

b

Cells were harvested 15 h after addition of IPTG. 

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