TABLE 1.
Specific activities of FGD expressed from recombinant plasmids in E. coli BL21(DE3)
E. coli BL21(DE3) carrying: | Mean FGD sp
act (μmol of F420 reduced/min/mg of protein) ± SD
|
|||
---|---|---|---|---|
2 ha
|
15
hb
|
|||
No IPTG | With IPTG | No IPTG | With IPTG | |
pEP7000 | 0.014 ± 0.002 | 0.018 ± 0.002 | 0.030 ± 0.003 | 0.011 ± 0.001 |
pEP5000 | 0.011 ± 0.002 | 0.010 ± 0.001 | 0.017 ± 0.002 | 0.005 ± 0.001 |
pEP3000 | 0.173 ± 0.018 | 0.254 ± 0.012 | 0.410 ± 0.014 | 0.187 ± 0.015 |
pEP1300 | 0.238 ± 0.014 | 0.277 ± 0.024 | 0.523 ± 0.033 | 0.224 ± 0.022 |
pEP1088 | 0 | 0 | 0.015 ± 0.001 | 0 |
pEP1300::Kanr | 0 | 0 | 0 | 0 |
Cells were harvested 2 h after addition of IPTG.
Cells were harvested 15 h after addition of IPTG.