Figure 3.

Effect of abemaciclib or TQB3616 on colony-formation- and cell cycle-associated proteins in breast cancer cell lines. (A and B) T47D cells and BT474 cells were treated with 0.1% DMSO, 0.5 µM abemaciclib, 1.0 µM abemaciclib, 1.5 µM abemaciclib, 0.5 µM TQB3616, 1.0 µM TQB3616, or 1.5 µM abemaciclib, and colony formation was assessed 2 to 3 weeks later as described in the Materials and methods section. Crystal violet staining of colonies is shown in the images. The means and standard deviations were determined on the basis of three independent experiments. (C and D) The T47D and BT474 cell lines were treated with abemaciclib or TQB3616 to inhibit cell cycle-related protein phosphorylation. ns, no statistical difference. **p<0.01, ****P<0.0001.