Fig 4.

Expression of parX from plasmid pSEVA224 complements the phenotype of P. putida KT2440 with the triple deletion ΔpmΔparX. (A) Strains containing the pSEVA224 plasmid encoding the adapter protein ParX (cyan) or empty plasmids (control, light, and medium gray) were grown in a 96-well plate with (A) KB medium supplemented with 1 mM 2′2-bipyridyl or (B) CAA medium. Media contained 0.5 mM IPTG for gene expression and kanamycin (50 mg/mL) for plasmid maintenance. Growth was started with an initial OD₆₀₀ of 0.1, cultures were continuously shaken, and the OD₆₀₀ was monitored for 20 h. (B) Growth rates were calculated from the exponential growth phase and are shown in Fig. S4. Mean values were calculated from a minimum of three biological replicates.