Fig 1.
PegIFNα treatment upregulates TRIM22 expression. HepAD38 and HepG2.2.15 cells were plated at a medium density of 1 × 105 cells and, after attachment, treated with 1,000 IU/mL PegIFNα or with sterile PBS for 24 hours. (A and B) mRNA level of TRIM-family genes determined by qRT-PCR in (A) HepAD38 and (B) HepG2.2.15 cells. (C and D) (Left) Western blot and (right) quantification of TRIM22 protein in (C) HepAD38 and (D) HepG2.2.15 cells. Data from a representative experiment of two or three independent replicates are shown. Unpaired Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001.