Fig 5.
PacC directly regulates transcription of ACT biosynthetic genes. (A) Physical position of ACT biosynthetic cluster. Red arrows correspond to the genes having a PacC binding motif 1 kb upstream of the first ATG codon. (B) Validation of the interaction between PacC and putative binding motif in the promoter of three ACT biosynthetic genes by electrophoretic mobility shift assay (EMSA). A schematic diagram shows the predicted binding motif of PacC in the promoter region of ACT biosynthetic genes (upper panel). The promoter of each gene was incubated with purified PacCZF-MBP or MBP at 28°C for 20 min.