Fig 5.

4CMenB vaccination of mice elicits cross-reactive antibodies that bind the surface of multiple strains of N. gonorrhoeae. Bacteria of the indicated strain background were incubated with a 1:10 dilution of pooled serum from 4CMenB-vaccinated or alum-treated mice or mouse IgG isotype control. Bacteria were fixed, processed for imaging flow cytometry, and fluorescence index calculated as in Fig. 1. Bars indicate the mean ± SD of three independent experiments, with each biological replicate as one data point. *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.001, and ****P ≤ 0.0001 by ordinary one-way ANOVA with Tukey’s multiple comparison’s test.