TABLE 1.
Antibodies used in this study
| Target | Antibody/clone name | Species, isotype | Labela | Concentration (mg/ml) | Source | Usea | Comments |
|---|---|---|---|---|---|---|---|
| 2C7 | Anti-N. gonorrhoeae LOS | IgG3 | (-) | 0.28 mg/ml | S. Ram, UMass | IFC, C3 deposition | Hybridoma supernatant |
| 6B4 | Anti-N. gonorrhoeae LOS | IgM, κ | (-) | 0.33 mg/ml | S. Ram, UMass | IFC, SBA | Hybridoma supernatant |
| CD11b, human | M1/70 | Rat IgG2b, κ | PE | 0.2 mg/ml | Biolegend | Flow cytometry | Catalog # 101207 |
| N. gonorrhoeae | Rabbit | (-) | 4-5 mg/ml | Meridian | IFC, SBA, OPKA | Catalog #B65111R | |
| IgG, rabbit | AffiniPure, Fc-fragment specific | Goat | AF488 | 1.5 mg/ml | Jackson Immuno Research | IFC | Catalog # 111-545-008 |
| IgG, mouse | Fcy fragment specific | Goat | AF488 | 1.5 mg/ml | Jackson Immuno Research | IFC | Catalog # 115-545-164 |
| IgM, mouse | µ-Chain specific | Goat | AF647 | 1.4 mg/ml | Jackson Immuno Research | IFC | Catalog # 115-606-075 |
| C3, human | Goat IgG | FITC | 7 mg/ml | MP Biomedical | IFC | Catalog # 55167 | |
| Isotype control for mouse IgG | E1D5H | Mouse IgG3 | FITC | 2.5 mg/ml | Cell Signaling Technology | IFC | Catalog # 37988 |
| Isotype control for mouse IgM | Clone MM-30 | Mouse IgMκ | APC | 2-3 mg/ml | Biolegend | IFC | Catalog # 401630 |
| Isotype control for goat anti-human C3 | Goat IgG | (-) | 1 mg/ml | R&D Systems | IFC | Catalog # AB-108-C |
a
AF, AlexaFluor; APC, allophycocyanin; FITC, fluorescein isothiocyanate; IFC, imaging flow cytometry; OPKA, opsonophagocytic killing; PE, phycoerythrin; SBA, serum bactericidal activity.