TABLE 2.
13C and 1H NMR chemical shifts (δ, ppm) of the K70 CPS of A. baumannii SGH0807 and a modified polysaccharide (MPS) obtained by Smith degradation of the CPS a
| Sugar residue | C-1 H-1 |
C-2 H-2 |
C-3 H-3 |
C-4 H-4 |
C-5 H-5 |
C-6 H-6 |
|---|---|---|---|---|---|---|
| CPS
b
→3)-α-d-GalpNAcA-(1→ A |
99.8 5.13 |
49.8 4.45 |
76.8 4.20 |
75.2 4.63 |
72.4 4.62 |
175.7 |
| →3)-α-l-FucpNAc-(1→ B |
100.1 4.88 |
49.8 4.30 |
74.6 4.10 |
72.3 3.68 |
68.2 3.99 |
16.5 1.28 |
| →3)-β-d-FucpNAc-(1→ C |
102.9 4.59 |
53.2 3.78 |
77.5 3.91 |
68.3 3.99 |
72.4
3.76 |
16.8 1.19 |
| α-l-FucpNAc-(1→ D |
97.9 5.48 |
50.8 4.09 |
68.9 3.91 |
72.4 3.76 |
68.3 4.09 |
16.5 1.19 |
| MPS
c
→3)-α-d-GalpNAcA-(1→ A |
100.0 5.07 |
49.3 4.27 |
77.0 4.04 |
70.9 4.54 |
72.1 4.44 |
n.f. |
| →3)-α-l-FucpNAc-(1→ B |
100.0 4.92 |
49.9 4.29 |
74.9 4.05 |
71.9 3.72 |
68.4 4.07 |
16.6 1.19 |
| →3)-β-d-FucpNAc-(1→ C |
103.4 4.53 |
53.1 3.92 |
77.2 3.82 |
68.4 4.07 |
72.3
3.78 |
16.9 1.26 |
a
1H NMR chemical shifts are italicized.
b
The following are the chemical shifts for the N-acetyl groups: δH 1.91–2.03 (Me); δC 23.6–23.8(Me) and 174.1–174.8 (CO).
c
The following are the chemical shifts for the N-acetyl groups: δH 1.91–2.08 (Me); δC 23.8–23.9(Me) and 174.7–175.1 (CO)b.