S. parasanguinis colonizes the mammalian lung. (A) Rat infection scheme. Rats were inoculated intranasally with or without S. parasanguinis (SP) or 1 mM nitrite. Eight hours later, rats were inoculated with or without P. aeruginosa (PA) or nitrite. After 24 hours, rats were sacrificed for downstream experiments. (B) S. parasanguinis CFU recovered from rats 24 hours post infection. (C) P. aeruginosa CFU recovered from rats 24 hours post infection. n = 8–11, ns, P > 0.05, (Kruskal-Wallis test, Dunett’s post hoc test).