TABLE 2.
RT-qPCR primers
| Species | Primer | Sequence |
|---|---|---|
| Porcine | IFN-β-F | GCTAACAAGTGCATCCTCCAAA |
| IFN-β-R | AGCACATCATAGCTCATGGAAAGA | |
| ISG15-F | CCTGTTGATGGTGCAAAGCT | |
| ISG15-R | TGCACATAGGCTTGAGGTCA | |
| GAPDH-F | ACATGGCCTCCAAGGAGTAAGA | |
| GAPDH-R | GATCGAGTTGGGGCTGTGACT | |
| TGEV | Nucleocapsid Protein-F |
ACGCTTGGTAGTCGTGGTG |
| Nucleocapsid Protein-R |
GAATTGTTGCCTGCCTCT | |
| Feline | IFN-β-F | GAAGGAGGAAGCCATATTGGT |
| IFN-β-R | CTCCATGATTTCCTCCAGGAT | |
| ISG15-F | TCCTGGTGAGGAACCACAAGGG | |
| ISG15-R | TTCAGCCAGAACAGGTCGTC | |
| 18s-F | CGGCTACCACATCCAAGGAA | |
| 18s-R | GCTGGAATTACCGCGGCT | |
| FIPV | Nucleocapsid Protein-F |
TCGTGGTGACTCTAA |
| Nucleocapsid Protein-R |
GATGGAACGCATTCA |