FIGURE 3.
FAD fluorescence lifetimes of MCF7 cells were altered with different pyruvate concentrations of the culture media. Elevated pyruvate concentration resulted in an increase in the bound FAD fraction (α 1 ), and bound FAD lifetime (τ 1 ). (A) Representative FAD τ m images of cancer cells exposed to different pyruvate concentrations, Py, pyruvate; scale bar = 60 μm (B) FAD α 1 (C) FAD τ 1 (D) FAD τ 2 (E) FAD τ m (F) NAD(P)H intensity (G) FAD intensity. *p < 0.05, ***p < 0.001 for two-sided Wilcoxon test with Bonferroni correction for multiple comparisons. Substrates in each media: Control (25 mM glucose +1 mM pyruvate), Pyruvate (no glucose + no glutamine +0/10/20/50 mM pyruvate).