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Purification of a 70-kDa protein that binds boxA RNA. Fractions containing boxA-binding activity were pooled after DE52 (lane 3), phenyl-Sepharose (lane 4), and poly(U)-agarose (lane 5) column chromatography and were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie blue. Lane 1 contains protein molecular mass standards (97.4, 66.2, 45.0, 31.0, and 21.5 kDa from top to bottom), and lane 2 contains E. coli S100 extract.
