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. 2023 Aug 7;18(12):1463–1473. doi: 10.1038/s41565-023-01483-3

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© The Author(s) 2023, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, Confocal image of hiPSC cysts embedded in DyNAtrix [+RGD] that was crosslinked in serum-free mTeS1 medium. Cells were stained for OCT4 (pluripotency), phalloidin (F-actin) and DAPI (nuclei). b, Confocal image of MDCK cysts grown in DyNAtrix [+RGD] using heat-activated 18 nt SRCs as the crosslinker library. The cells were modified to express mNeonGreen-labelled E-cadherin and mScarlet-labelled podocalyxin. c, Confocal image of trophoblast organoids cultured in DyNAtrix [+RGD]. Cells were immunofluorescently stained for GATA3, E-cadherin, ENDOU and DAPI (nuclei). d, Representative microscopy images of hiPSC cysts grown in DyNAtrix [+RGD] compared with Matrigel. A comparison with DyNAtrix [−RGD] is shown in Supplementary Fig. 15. e, Representative confocal microscopy images of hiPSC cysts grown in DyNAtrix [+RGD] compared with Matrigel (day 7). Top: cross-sections. Bottom: projection image of a cyst in DyNAtrix [+RGD]. f, Representative bright-field images of trophoblast organoids grown in DyNAtrix [+RGD]/[–RGD] versus Matrigel. The red arrows mark organoids. g, Confocal microscopy images of a trophoblast organoid in DyNAtrix [+RGD] stained for TEAD4, E-cadherin, GCM1 and DAPI (nuclei) (day 7). A comparison with organoids in Matrigel is shown in Supplementary Fig. 21.