Table 2.

Cryo-EM data collection, refinement and validation statistics

	α5V3-DZP (EMDB-16058), (PDB 8BHK)	α5V3-APO (EMDB-16005), (PDB 8BEJ)	α5V3-DMCM (EMDB-16060), (PDB 8BHM)	α5V3-RO4938581 (EMDB-16068), (PDB 8BHS)	α5V3-L655708 (EMDB-16063), (PDB 8BHO)	α5V3-RO154513 (EMDB-16051), (PDB 8BHB)
Data collection and processing
Magnification	130,000	130,000	92,000	130,000	130,000	130,000
Voltage (kV)	300	300	200	300	300	300
Electron exposure (e− Å−2)	57.8	49.27	23.1	55.15	54.24	53.28
Defocus range (μm)	0.6–2.7	0.8–2.2	1.2–2.7	1.0–2.5	1.0–2.5	1.0–2.5
Pixel size (Å)	1.07	0.652	0.106	1.05	1.05	0.652
Symmetry imposed	C5	C5	C5	C5	C5	C5
Initial particle images (no.)	133,526	39,108	181,736	215,822	194,357	42,434
Final particle images (no.)	47,605	9,041	53,252	43,386	21,342	18,185
Map resolution (Å)	3.3	3.28	2.95	3.24	2.93	2.54
FSC threshold	0.143	0.143	0.143	0.143	0.143	0.143
Map resolution range (Å)	3.2–4.6	3.1–4.4	3.1–3.7	2.8–4.4	2.7–4.6	2.4–3.8
Refinement
Initial model used (PDB code)	4COF	4COF	4COF	4COF	4COF	4COF
Model resolution (Å)	3.3	3.28	2.95	3.24	2.93	2.54
FSC threshold	0.143	0.143	0.143	0.143	0.143	0.143
Model resolution range (Å)	3.2–4.6	3.1–4.4	3.1–3.7	2.8–4.4	2.7–4.6	2.4–3.8
Model composition
Non-hydrogen atoms	13,750	13,650	13,765	13,755	13,775	13,835
Protein residues	1,680	1,680	1,680	1,680	1,680	1,680
Ligands	2	1	2	2	2	2
B factors (Å2)
Protein	52	131	134	109	108	80
Ligand	52, 48	144, N/A	140, 119	122, 124	117, 87	96, 67
R.m.s. deviations
Bond lengths (Å)	0.001	0.002	0.001	0.002	0.001	0.002
Bond angles (°)	0.345	0.355	0.335	0.379	0.360	0.355
Validation
MolProbity score	0.88	1.07	0.96	1.32	1.05	1.00
Clashscore	1.47	2.58	1.98	5.87	2.60	2.27
Poor rotamers (%)	0.00	0.00	0.00	0.00	0.00	0.00
Ramachandran plot
Favored (%)	98.20	97.9	99.1	98.80	99.10	99.10
Allowed (%)	1.80	2.10	0.90	1.20	0.90	0.90
Disallowed (%)	0.00	0.00	0.00	0.00	0.00	0.00