Extended Data Fig. 6. Small molecule inhibitors alter AR proteomic interactions with Mediator.
a) Representative (n = 2) western blot showing expression of FLAG-MTID-AR or FLAG-MTID-Y22toS proteins in LNCaP cells with antibodies for AR. Biotin-dependent labeling is shown with Streptavidin antibodies (Strep) and GAPDH and Ponceau staining are shown as loading controls. EV indicates the empty vector expressing FLAG-MTID. b) BioID MS of LNCaP MTID-AR-WT interaction with Mediator complex. Colour indicates strength of interaction from FLAG, LogFC10 Tmean of intensity. c) TMean SAINTq intensity of total mediator interactions were compared across LNCaP MTID-AR-WT with DMSO or treated cells with small molecule inhibitors.