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. 2023 Dec 12;14:8217. doi: 10.1038/s41467-023-43001-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Analysis of the L1 retrotransposition efficiency in control or cGAS-knockout stress-induced premature senescent (SIPS) HeLa cells. WT or cGAS-knockout cells transfected with the pEGFP-LRE3 reporter were treated with 10 µg/mL etoposide for 20 min on day 2 post-transfection, and harvested for FACS analysis on day 9. n = 3 independent experiments. b Analysis of L1 retrotransposition efficiency in control or cGAS-overexpressed SIPS cells. n = 6 independent experiments. c Analysis of CHK2 and cGAS phosphorylation levels in senescent HeLa cells. d Subcellular localization of phosphorylated cGAS in senescent HeLa cells. e Analysis of the subcellular localization of phosphorylated cGAS in X-ray induced senescent IMR90-hTERT cells. f Analysis of the subcellular localization of phosphorylated cGAS in X-ray induced senescent HCA2-hTERT cells. For (e) and (f), cells were irradiated by 15 Gy X-ray and lysed for protein extraction and Western blot analysis on day 9 post irradiation. Data are presented as mean values ± s.d. Student’s t test was performed for (a) and (b). All statistical test used were two-sided. All experiments were repeated three times.