Fig. 3. Metabolic genes signature in HUVECs upon silencing ANGPTL4 in the presence and absence of hypoxia mimetic CoCl₂.

HUVECs were transfected for 6 h with siRNA against ANGPTL4 or NS control and then treated with CoCl₂ or vehicle control, cells were harvested 60 h post-transfection for RNA-seq analysis (n = 3 biological replicates). a Venn diagram showing the differentially expressed common genes between siANGPTL4 and NS treated with or without hypoxia mimetic CoCl₂. b KEGG-GO pathway analysis of 1056 differentially and significantly upregulated (red bars) and 868 downregulated (blue bars) genes in siANGPTL4 vs NS treated with CoCl₂ (significance for DGE was determined with Limma’s decideTests). c Heatmap representing the transcript levels of indicated pathway genes. Color key: red, high correlation; blue, low correlation. d Pathway map representing changes in transcript levels of genes in central carbon metabolism in siANGPTL4 vs NS treated with CoCl₂. Color key: red, upregulated genes; blue: downregulated, gray: unchanged. Exact p values are shown for each comparison. Source data are provided as a Source data file.