Figure 6.

Assessment of off-target editing

On-target or off-target editing at top ONE-seq-nominated or in-silico-nominated candidate sites calculated as the proportion of aligned sequencing reads with alteration of ≥1 base pair within the target sequence (ranging from the position proximal to the pegRNA nick site to the distal position spanned by the RTT sequence, capturing both single-nucleotide substitutions and indels) in PAH c.1222C>T homozygous HuH-7 cells that underwent plasmid transfection with the lead pegRNA/ngRNA (P56/N19) combination (n = 3 treated and 3 untreated biological replicates; y axis is log₁₀ scale). Sites with unsuccessful sequencing are omitted. Refer to Table S4 for candidate site sequences and numerical values.