Figure 3.
Decreased stem cell populations in G-SmoAMPK tumors
(A) Expression of indicated stem cell markers in the indicated genotypes, projected onto the disaggregated UMAPs. Hes1, Sox2, Olig2 and Vim were significantly lower in G-SmoAMPK tumors, while Nes was not significantly different.
(B) Dot plot in which the fractions of cells in each genotype that express the marker are represented by dot size. This panel presents the same data as panel (A), visualized using a different method. Hierarchical clustering show relatively similar variation across genotypes for the group of Olig2 and Vim and for the group of Sox2 and Hes1.
(C) Representative OLIG2/SOX10 IF in sagittal sections of tumors of indicated genotype.
(D) Quantification of OLIG2/SOX10 IF as in (C) in replicate samples of each genotype.
(E) Quantification of Yfp+ glial cells in the indicated genotypes. In panels (A), (B), (D) and (E), the p values were determined Student’s t test. In panel (C), bars = 2 mm in the left panel and 100 μm in the right panel.