REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
OLIG2	Cell Marque	Cat.# 387R-14
Sox10	Cell Signaling Technology	Cat.# 7833S
PRB	Cell Signaling Technology	Cat.# 8957
NeuN	Millipore	Cat.# MAB377; RRID: AB_2298772
Ki67	Cell Signaling Technology	Cat.# 12202S
NeuroD1	Abcam	Cat.# ab213725; RRID: AB_2801303
p4EBP1	Cell Signaling Technology	Cat.# 2855
Critical commercial assays
Papain Dissociation System	Worthington Biochemical	Cat.# LK003150
Barcoded Seq B Drop-seq beads	ChemGenes	Cat.# Macosko-2011-10(V+)
PDMS device	FlowJEM	Request: “Drop-Seq Device with Aquapel Coating”
Deposited data
scRNA-seq raw and analyzed data	This paper	GEO: GSE150579
scRNA-seq raw and analyzed data	This paper	GEO: GSE190297
Mouse: GFAP-Cre: FVB-Tg(GFAP-cre)25Mes/J	The Jackson Laboratory	RRID:IMSR_JAX:004600
Mouse: Prkaa1loxP: Prkar1atm1.2Lsk/J	The Jackson Laboratory	RRID:IMSR_JAX:036801
Mouse: Prkaa2loxP: Prkaa2tm1.1Sjm/J	The Jackson Laboratory	RRID:IMSR_JAX:014142
Mouse: SmoM2: Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J	The Jackson Laboratory	RRID:IMSR_JAX:005130
Mouse: C57BL/6:	The Jackson Laboratory	RRID:IMSR_JAX:000664
Software and algorithms
Seurat R package version 3.1.1	Satija Lab	https://cran.r-project.org/src/contrib/Archive/Seurat/Seurat_3.1.1.tar.gz
Harmony algorithm	Korsunsky et al.61	https://github.com/immunogenomics/harmony
Tissue Studio 4.4.2	Definiens	Build 60765 x64